Kidney

kidney_he_02.jpg kidney_pen_02.jpg
Kidney_H&E_02.svs 
Kidney_H&E_02.png
Kidney_Pen_02.svs 
Kidney_Pen_02.png

Collagen is more effectively visualized using the pentachrome stain kit at the medulla due to the contrast of the green/blue staining of the supporting stroma. The pentachrome red staining of the muscles helps to differentiate between the type of blood vessels present as the arterial vessels are surrounded by muscle and the venous blood vessels are not.

Previous Attempts

kidney_he_01.jpg kidney_pen_01.jpg
Kidney_H&E_01.svs 
Kidney_H&E_01.tif
Kidney_Pen_01.svs 
Kidney_Pen_01.tif

Collagen is more effectively visualized using the pentachrome stain kit at the medulla due to the contrast of the green/blue staining of the supporting stroma. The pentachrome red staining of the muscles helps to differentiate between the type of blood vessels present as the arterial vessels are surrounded by muscle and the venous blood vessels are not. The intensity of the pentachrome red stains on the cytoplasm reduced the visibility of the nuclei. Further, the intensity of the red blood cells stained red obscures the visibility of cellular borders.