Femur

Bone Marrow

femur

Control_Femur_HE_03.svs
Diabetic_Femur_HE_03.svs
Femur Tissue_HE and Pen 03.png

Control_Femur_Pen_03.svs
Diabetic_Femur_Pen_03.svs
Eye_Tissue_HE and Pen 03.tiff

Femoral Cartilage

femur

?Control_Femur_HE_03.svs
?Diabetic_Femur_HE_03.svs
Femur Tissue_HE and Pen 03.png

?Control_Femur_Pen_03.svs
?Diabetic_Femur_Pen_03.svs
Eye_Tissue_HE and Pen 03.tiff

The blue colour in the pentachrome stain kit represents mucin and indicates the progression of bone remodelling. The pentachrome stain kit helps to visualize the bone material before ossification. Chondroids and the matrix are also well differentiated and visualized. Further, striations of the muscles, bone, and cartilage are well visualized with the pentachrome stain kit.

Control Tissues

Epiphysis

femur_he_02.JPG femur_pen_02.JPG
Femur_Diaphysis_H&E_02.svs 
Femur_Epiphysis_H&E_02.png
Femur_Diaphysis_Pen_02.svs 
Femur_Epiphysis_Pen_02.png

The blue colour in the pentachrome stain kit represents mucin and indicates the progression of bone remodelling. The pentachrome stain kit helps to visualize the bone material before ossification. Chondroids and the matrix are also well differentiated and visualized. Further, striations of the muscles, bone, and cartilage are well visualized with the pentachrome stain kit.

Previous Attempts

Femur

Diaphysis

femur_diaphysis_he_01.JPG femur_diaphysis_pen_01.JPG
Femur_Diaphysis_H&E_01.svs 
Femur_Diaphysis_H&E_01.tif
Femur_Diaphysis_Pen_01.svs 
Femur_Diaphysis_Pen_01.tif

Epiphysis

femur_epiphysis_he_01.JPG femur_epiphysis_pen_01.JPG

Femur_Epiphysis_H&E_01.svs 
Femur_Epiphysis_H&E_01.tif

Femur_Epiphysis_Pen_01.svs 
Femur_Epiphysis_Pen_01.tif

The blue colour in the pentachrome stain kit represents mucin and indicates the progression of bone remodelling. The pentachrome stain kit helps to visualize the bone material before ossification. Chondroids and the matrix are also well differentiated and visualized. Further, striations of the muscles, bone, and cartilage are well visualized with the pentachrome stain kit. The pentachrome stain kit’s colours are helpful, but the overall stain is not as crisp compared with the H&E stain.