Genetically modified (GM) mouse lines are a critical resource for biomedical science. Unique mouse lines generated through pronuclear injection allow investigators to understand the specific role of a gene/protein in tissue development, cell function, and disease, or understand the regulation of gene expression. More and more, GM mouse lines are being used as preclinical models to understand the basis of many conditions including neurological diseases, cancer, diabetes, and arthritis.
The LRTGT has generated a number of GM mice by either ablating specific genes, inserting recombination sites, introducing novel gene isoforms, or through over-expressing miRNAs. We use traditional transgenic insertion approaches and CRISPR-Cas9 to create novel GM mice.
In addition to generating new lines, it has become very common for researchers to make new mouse lines by combining existing lines developed by a number of laboratories. Combining mouse lines is required for temporal and spatial deletion of genes, lineage tracing of cell throughout development, and understanding the combined effects of two or more genetic changes.
The LRTGT can import GM lines from all around the world and perform embryo rederivation or in vitro fertilization to remove any pathogens prior to entry into pathogen-free animal areas.