Instrument List

As of December 2022, we have available:

Protein Production Lab (which has a yearly membership fee)

ThermoFisher Quantstudio 3 qPCR: Real time PCR and protein thermal shift studies

Beckman Preparative Centrifuges and Ultracentrifuges: Separate cellular debris from protein-containing supernatant, fractionate complex samples, sucrose-density experiments

DeNovix microvolume spectrophotometer: Quantify protein/DNA samples, check OD of cell samples, collect absorbance/fluorescence spectra

Cytiva AKTA Go FPLCs: Fractionate and purify protein samples using ion exchange, affinity and size exclusion chromatography

Wyatt MiniDAWN MALS: Characterize the size and shape of your biomolecule

Agilent 1200 HPLC: Fractionate and purify peptides and smaller molecules

Avestin C3 Homogenizer: High-pressure cell lysis and sample homogenization

Sonicator (in M382): Nanoparticle dispersion, cell disruption, DNA shearing

BioRad ChemiDoc MP Imaging System: Brightfield and fluorescent imaging system

BioRad gel electrophoresis and blot equipment: SDS-PAGE gels and Western blots

ThermoFisher Savant speed vac: vacuum concentrator

Biophysical Analysis Lab (instruments usage is based on an hourly rate)

Octet R2-Biolayer Interferometry: High-throughput molecular interaction analysis

BioTek Synergy H1 Multi-Mode Plate Reader: Applications in fluorescence, luminescence, and UV-vis

BioXp 3200 DNA Printer: Automated workstation that builds and clones DNA fragments

Beckman XL-I Analytical Ultracentrifuge: Characterize size and shape of macromolecule or complex

TA Instruments Nano ITC: Characterization of molecular interactions

MicroCal VP-DSC Differential Scanning Calorimetor: Characterization of thermal stability of biomolecules

Jasco J-810 Circular Dichroism Spectropolarimeter: Protein secondary structure analysis and thermal characterization

LI-COR Odyssey Classic: Fluorescent imaging system

Victor3V Multilabel Counter: Multimode plate reader, luminescence, fluorescence polarization

Beckman Coulter Biomek i5: Automated liquid handling system

Cary UV-Vis Spectrophotometer: Quick measurements with temperature control

X-ray Crystallography Lab

Rigaku MicroMax 00H7 Rotating anode x-ray generator with high resolution CCD detector: Collect atomic-resolution structural data for your protein crystals

CrysAlis Pro workstation: Data collection and data processing software for protein and small molecule crystallography

Art Robbins Crystal Gryphon: High-throughput, high accuracy liquid handling robot for setting up your protein crystal screens

Formulatrix RockImager Plate Hotel and Imaging Robot: Image and score your protein crystals remotely, while they’re stored at a stable temperature

Crystallization suites: Choose from a variety of 96-well sparse matrix screens to crystallize your protein samples. Currently on site: JCSG+, PACT and MCSG screens 1-4.

Microscopes: Track the progress of your crystal plates, or use these stereomicroscopes while manipulating your crystals or seeding your crystal drops

Oxford NanoPore MinION Sequencer: Devices for DNA and RNA sequencing

BioNMR Lab

Bruker Avance NEO 600 MHz Spectrometer: Atomic-resolution 3D structural analysis

Bruker Avance NEO 600 MHz Spectrometer with SampleJet: High-throughput analysis, including metabolomic research

Mass Spectrometry Labs

Thermo Orbitrap Elite Velos Pro with Waters MClass UPLC, and Agilent 1100

Thermo QExactive Plus, with Waters MClass UPLC

Thermo Eclipse, with Vanquish Neo

AB SCIEX TOF/TOF 5800 System (MALDI)