Peter Stathopulos

Peter Stathopulos

Assistant Professor

PH.D. University of Waterloo 
M.Sc. The University of Western Ontario 
B.Sc. University of Waterloo 
Office:  Medical Sciences Building, Room 232
p. 519.661.2111 x. 83238
Lab: Medical Sciences Building, Room 219
p. 519.661.2111 x. 82812
f. 519.661.3827
e. pstatho@uwo.ca

See Publications by Peter Stathopulos on PubMed:

https://www.ncbi.nlm.nih.gov/pubmed/?term=Stathopulos%20P

Laboratory Website:

http://publish.uwo.ca/~pstatho/StathopulosLab/content/en-ca/index.html

Biography:

Dr. Peter B. Stathopulos completed his B.Sc. at the University of Waterloo in Co-operative Biology. He later graduated with an M.Sc. from the University of Western Ontario in Pharmacology and Toxicology. His Ph.D. work at the University of Waterloo was in Biology, co-supervised through the Departments of Chemistry and Physics. He completed his Post-doctoral training in Structural Biology at the University of Toronto (Medical Biophysics) and at the Ontario Cancer Centre.

Our laboratory aims to apply structural biology approaches to revealing the molecular mechanisms driving calcium signaling processes in health and diseased states including heart disease, cancer and immunodeficiency. We integrate structural biology with a host of biophysical, chemical biology and live cell methodologies in an effort to understand the relationship between structure and function of critical calcium signaling proteins. Ultimately, we use this structure-function data for the rational identification of new drug binding targets with the potential to modulate these pathways, maintain health and treat disease. 

Keywords:
calcium signaling; molecular pharmacology; structural biology; solution nuclear magnetic resonance spectroscopy; live cell imaging; X-ray crystallography; calorimetry; protein folding; biomolecular thermodynamics; computational biology; protein structure and function; protein-protein interactions; protein allostery; ion channels; protein dynamics; light scattering; optical spectroscopy; heart disease; cancer; immunodeficiency

Selected Publications:

Novello, M.J., Zhu, J., Feng, Q., Ikura, M. and Stathopulos, P.B. (2018). Structural elements of stromal interaction molecule function. Cell Calcium. 73:88-94.

Zhu, J., Lu, X., Feng, Q. and Stathopulos, P.B. (2018). A charge sensing region in the STIM1 luminal domain confers stabilization-mediated inhibition of SOCE in response to S-nitrosylation. J Biol Chem. 293(23):8900-8911.

Fahrner, M., Stadlbauer, M., Muik, M., Rathmayr, P., Stathopulos, P.B., Ikura, M., Muller, N. and Romanin C. (2018). A novel mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state. Nat Commun. 9(1):825.

Enomoto, M., Nishikawa, T., Siddiqui, N., Chung, S., Ikura, M. and Stathopulos, P.B. (2017). From stores to sinks: structural mechanisms of cytosolic calcium regulation. Adv Exp Med Biol. 981:215-251.

Alevriadou, R., Shanmughapriya, S., Patel, A., Stathopulos, P.B., Madesh, M. (2017). Mitochondrial Ca2+ transport in the endothelium: Regulation by ions, redox signalling, and mechanical forces. J Royal Soc. Interface. 14(137).

Choi, Y.J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q. and Stathopulos, P.B. (2017). Targeting cysteine thiols for in vitro site-specific glycosylation of recombinant proteins. J Vis Exp. (128):e56302.

Choi, Y.J., Zhao, Y., Bhattacharya, M. and Stathopulos, P.B. (2017). Structural perturbations induced by Asn131 and Asn171 glycosylation converge within the EFSAM core and enhance stromal interaction molecule-1 mediated store operated calcium entry. Biochim Biophys Acta (BBA) – Mol Cell Res. 1864(6):1054-1063.

                        NOTE: Selected as journal issue cover art focus.

Lee, S.K., Shanmughapriya, S., Mok, M.C.Y., Dong, Z., Rajan, S., Junop, M., Madesh, M. and Stathopulos, P.B. (2016). The mitochondrial calcium uniporter is autoregulated by cation binding to a beta-grasp-like matrix domain. Cell Chem Biol. 23(9):1157-69.