PH.D. University of Hull, UK
M.Sc. University of Hull, UK
Office: Medical Sciences Building, Room 270
See Publications by Rithwik Ramachandran on PubMed
Visit: Dr. Ramachandran's website
My research program will focus on studying the molecular pharmacology of G Protein Coupled Receptors (GPCRs; focusing mainly on the Proteinase Activated Receptor family) and Transient Receptor Potential (TRP) ion channels. We use various techniques to understand cell-signaling responses to GPCRs and TRP channel activation at the molecular level and in vivo. These studies have relevance to various human diseases including cancer, cardiovascular diseases and inflammatory diseases such as colitis and asthma..
Techniques in the Lab: The lab will take a multidisciplinary approach and studies will range from understanding receptor/channel function at the molecular level to in vivo models of disease.
- Molecular cloning
- Calcium signaling/imaging
- BRET/FRET, designing and synthesizing novel biosensors
- Confocal, TIRF microscopy
- PCR, Western Blot, Immunostaining, ELISA
- Platelet signaling assays
- Vascular tissue assays
- Animals models of inflammation
Saifeddine M, El-Daly M, Mihara K, Bunnett NW, McIntyre P, Altier C, Hollenberg MD and Ramachandran R, GPCR mediated EGFR transactivation regulates TRPV4 action in the vasculature. British Journal Of Pharmacology. 2015 Jan 9. doi: 10.1111/bph.13072. [Epub ahead of print]
Mihara K, Ramachandran R, Renaux B, Saifeddine M and Hollenberg MD. Neutrophil Elastase and Proteinase-3 Trigger G Protein-biased Signaling through Proteinase-activated Receptor-1 (PAR1). Journal of Biological Chemistry, 2013 Nov 15;288(46):32979-90
Ramachandran R, Hyun E, Zhao L, Lapointe TK, Chapman K, Hirota CL, Ghosh S, McKemy D, Vergnolle N, Beck PL, Altier C and Hollenberg MD. Icilin attenuates inflammatory responses in mouse models of colitis. Proceedings of the National Academy of Sciences, 2013 Apr 30;110(18):7476-81.
Ramachandran R, Noorbakhsh F, DeFea K and Hollenberg MD. Targeting proteinase activated receptors: therapeutic potential and challenges. Nature Reviews Drug Discovery, 2012 Jan (11), 69-86.
Ramachandran R, Mihara K, Chung H, Renaux B, DeFea K, Bouvier M and Hollenberg MD. Neutrophil Elastase Acts as a biased agonists for Proteinase Activated Receptor-2 (PAR2). Journal of Biological Chemistry, 2011 Jul 15;286(28):24638-48.