Schulich school of Medicine and Dentistry logo Biological Mass Spectrometry Laboratory

Selected Publications

1.          Kuljanin, M., Dieters-Castator, D. Z., Hess, D. A., Postovit, L.-M. & Lajoie, G. A. Comparison of sample preparation techniques for large-scale proteomics. Proteomics 17, (2017).

2.          Sherman, S. E. et al. High Aldehyde Dehydrogenase Activity Identifies a Subset of Human Mesenchymal Stromal Cells with Vascular Regenerative Potential. Stem Cells (2017). doi:10.1002/stem.2612

3.          Park, D. H., Wang, L., Pittock, P., Lajoie, G. & Whitehead, S. N. Increased Expression of GM1 Detected by Electrospray Mass Spectrometry in Rat Primary Embryonic Cortical Neurons Exposed to Glutamate Toxicity. Anal. Chem. 88, 7844–7852 (2016).

4.          Sun, W. et al. An Effective Approach for Glycan Structure De Novo Sequencing From HCD Spectra. IEEE Trans. Nanobioscience 15, 177–184 (2016).

5.          Zebian, N. et al. Comprehensive analysis of flagellin glycosylation in Campylobacter jejuni NCTC 11168 reveals incorporation of legionaminic acid and its importance for host colonization. Glycobiology 26, 386–397 (2016).

6.          Szyszka-Mroz, B., Pittock, P., Ivanov, A. G., Lajoie, G. & Huner, N. P. A. The Antarctic Psychrophile Chlamydomonas sp. UWO 241 Preferentially Phosphorylates a Photosystem I-Cytochrome b6/f Supercomplex. Plant Physiol. 169, 717–736 (2015).

7.          Hanuszkiewicz, A. et al. Identification of the flagellin glycosylation system in Burkholderia cenocepacia  and the contribution of glycosylated flagellin to evasion of human innate immune responses. J. Biol. Chem. 289, 19231–19244 (2014).

8.          He, L., Xin, L., Shan, B., Lajoie, G. A. & Ma, B. GlycoMaster DB: software to assist the automated identification of N-linked glycopeptides by tandem mass spectrometry. J. Proteome Res. 13, 3881–3895 (2014).

9.          Turowec, J. P. et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol. Cell. Proteomics 13, 1184–1197 (2014).

10.        Liu, H. et al. A method for systematic mapping of protein lysine methylation identifies functions for HP1beta in DNA damage response. Mol. Cell 50, 723–735 (2013).

11.        Chan, B. P. H. et al. On the catalysis of calcium oxalate dihydrate formation by osteopontin peptides. Colloids Surf. B. Biointerfaces 96, 22–28 (2012).

12.        Hughes, C. et al. Mass spectrometry-based proteomic analysis of the matrix microenvironment in pluripotent stem cell culture. Mol. Cell. Proteomics 11, 1924–1936 (2012).

13.        Peng, Y. et al. Human fibroblast matrices bio-assembled under macromolecular crowding support stable propagation of human embryonic stem cells. J. Tissue Eng. Regen. Med. 6, e74-86 (2012).

14.        Zhang, J. et al. PEAKS DB: de novo sequencing assisted database search for sensitive and accurate  peptide identification. Mol. Cell. Proteomics 11, M111.010587 (2012).

15.        Duncan, K. E. et al. Discovery and characterization of a nonphosphorylated cyclic peptide inhibitor of the peptidylprolyl isomerase, Pin1. J. Med. Chem. 54, 3854–3865 (2011).

16.        Grohe, B. et al. Cooperation of phosphates and carboxylates controls calcium oxalate crystallization in ultrafiltered urine. Urol. Res. 39, 327–338 (2011).

17.        Hughes, C. S., Nuhn, A. A., Postovit, L. M. & Lajoie, G. A. Proteomics of human embryonic stem cells. Proteomics 11, 675–690 (2011).

18.        Hughes, C. S., Radan, L., Betts, D., Postovit, L. M. & Lajoie, G. A. Proteomic analysis of extracellular matrices used in stem cell culture. Proteomics 11, 3983–3991 (2011).

19.        O’Young, J. et al. Matrix Gla protein inhibits ectopic calcification by a direct interaction with hydroxyapatite crystals. J. Am. Chem. Soc. 133, 18406–18412 (2011).

20.        Azzopardi, P. V et al. Roles of electrostatics and conformation in protein-crystal interactions. PLoS One 5, e9330 (2010).

21.        Baht, G. S. et al. Phosphorylation of Ser136 is critical for potent bone sialoprotein-mediated nucleation of hydroxyapatite crystals. Biochem. J. 428, 385–395 (2010).

22.        Hughes, C. S., Postovit, L. M. & Lajoie, G. A. Matrigel: a complex protein mixture required for optimal growth of cell culture. Proteomics 10, 1886–1890 (2010).

23.        Hughes, C., Ma, B. & Lajoie, G. A. De novo sequencing methods in proteomics. Methods Mol. Biol. 604, 105–121 (2010).

24.        Iosef, C. et al. Insulin-like growth factor binding protein-6 (IGFBP-6) interacts with DNA-end binding protein Ku80 to regulate cell fate. Cell. Signal. 22, 1033–1043 (2010).

25.        Sui, R., Liu, S., Lajoie, G. A. & Charpentier, P. A. Preparing titania aerogel monolithic chromatography columns using supercritical carbon dioxide. J. Sep. Sci. 33, 1604–1609 (2010).

26.        Beasley, F. C. et al. Characterization of staphyloferrin A biosynthetic and transport mutants in Staphylococcus aureus. Mol. Microbiol. 72, 947–963 (2009).

27.        Bendall, S. C. et al. An enhanced mass spectrometry approach reveals human embryonic stem cell growth factors in culture. Mol. Cell. Proteomics 8, 421–432 (2009).

28.        Cheung, J., Beasley, F. C., Liu, S., Lajoie, G. A. & Heinrichs, D. E. Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus. Mol. Microbiol. 74, 594–608 (2009).

29.        Bendall, S. C. et al. Prevention of amino acid conversion in SILAC experiments with embryonic stem cells. Mol. Cell. Proteomics 7, 1587–1597 (2008).

30.        Liu, S. et al. Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 22, 197–203 (2008).

31.        Ballard, J. N. M., Lajoie, G. A. & Yeung, K. K.-C. Selective sampling of multiply phosphorylated peptides by capillary electrophoresis for electrospray ionization mass spectrometry analysis. J. Chromatogr. A 1156, 101–110 (2007).

32.        Zhang, H., Hunter, G. K., Goldberg, H. A., Lajoie, G. A. & Yeung, K. K.-C. An integrated procedure of selective injection, sample stacking and fractionation of phosphopeptides for MALDI MS analysis. Anal. Chim. Acta 581, 268–280 (2007).

 

Photos by Hannah O'Gorman